Difference between revisions of "Part:BBa K5280401"

 
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<partinfo>BBa_K5280401 short</partinfo>
 
<partinfo>BBa_K5280401 short</partinfo>
  
 
PcolE48 is a promoter to which LexRO (BBa_K5280129) binds and represses the expression of genes driven by the promoter. The promoter mainly consists of two parts: ColE promoter, which is a strong promoter, and LexA(408)-operator, which is the binding site of LexRO.
 
PcolE48 is a promoter to which LexRO (BBa_K5280129) binds and represses the expression of genes driven by the promoter. The promoter mainly consists of two parts: ColE promoter, which is a strong promoter, and LexA(408)-operator, which is the binding site of LexRO.
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<!-- -->
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=== Sequence and Features ===
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<partinfo>BBa_K5280401 SequenceAndFeatures</partinfo>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===
  
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<!-- -->
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PcolE408 basically has 2 parts:
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<span class='h3bb'>Sequence and Features</span>
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* ColE promoter, which is used to drive the expression of downstream genes;
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<partinfo>BBa_K5280401 SequenceAndFeatures</partinfo>
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* LexA(408) operator, which is used to provide the regulatory site for LexRO.
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The basic working principle is illustrated in the figure below. For more information, you can refer to the part page <partinfo>BBa_K5280129</partinfo>.
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    <div class="figure-box">
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        <img
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            src="https://static.igem.wiki/teams/5280/registry/lexro/lexro-mechanism-ver-2.png"
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            style="width:900px; display:block; margin:0;"
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            alt="In the experiments, E. coli transformed with an empty vector were used as negative controls."
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        >
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        <p>
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            This figure shows the mechanism of the eLightOn system (LexRO based regulation system).
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        </p>
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    </div>
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Revision as of 13:35, 29 September 2024


PcolE408

PcolE48 is a promoter to which LexRO (BBa_K5280129) binds and represses the expression of genes driven by the promoter. The promoter mainly consists of two parts: ColE promoter, which is a strong promoter, and LexA(408)-operator, which is the binding site of LexRO.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]