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| − | <!-- DON'T DELETE --><partinfo>BBa_I731012 EndReviews</partinfo>
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| − | Experiment : checking BBa_I731012
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| − | #Picked and cultured the following stab and glycerol stocks in 2mL of LB:
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| − | ##LB-Amp, BBa_I731012, (stab, received from iGEM)
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| − | ##LB-Amp+0.2%Glu, BBa_K084012(plac+rbs+LuxI(no LVA)), (XL10G)
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| − | ##LB-Amp+0.2%Glu, BBa_K084007(plac+rbs+LasI(no LVA)), (XL10G)
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| − | #Pre-Cultured at 37°C for 12h.
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| − | #Added 6.25% each of the pre-cultures to new LB medium.
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| − | ##LB-Amp, BBa_T9002, BBa_K084012(plac+rbs+LuxI(no LVA)), BBa_K084007(plac+rbs+LasI(no LVA))
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| − | #Cultured at 37°C for 12h
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| − | #Mixed the sender cells BBa_K084012 and BBa_K084007 both with BBa_I1731012 at a 1:1 ratio.
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| − | ##BBa_I1731012 alone.
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| − | ##BBa_K084012:BBa_I1731012=1:1
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| − | ##BBa_K084007:BBa_BBa_I1731012=1:1
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| − | #Culture 12h and observe results(follow picture).
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| − | *Funakoshi, UVGL-58 : long wave(365 nm)
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| − | <html><a href="https://static.igem.org/mediawiki/igem.org/0/0e/BBa_I731012-check.png" target="_blank"> <img src="https://static.igem.org/mediawiki/igem.org/0/0e/BBa_I731012-check.png" width="400" height="300"></a></html>
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Revision as of 22:07, 21 October 2009
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