Difference between revisions of "Part:BBa K5387000"
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ALOX12B is a gene found on human chromosome 17 and encodes for the enzyme 12R-LOX. The enzyme is an example of an arachidonate lipoxygenase, which is a family of enzymes that has arachidonic acid as a substrate. The enzymes will add an oxygen molecule to the substrate producing a fatty acid hydroperoxide, which later on acts as an important signaling molecule. For the case of 12R-LOX, 12R-hydroperoxyeicosatetraenoic acid (12R-HPETE) is produced when adding an oxygen molecule. 12R-HPETE is then transformed into a signaling molecule important for lipid formation in the epidermis, which serves a necessary role for preventing skin dehydration [1]. | ALOX12B is a gene found on human chromosome 17 and encodes for the enzyme 12R-LOX. The enzyme is an example of an arachidonate lipoxygenase, which is a family of enzymes that has arachidonic acid as a substrate. The enzymes will add an oxygen molecule to the substrate producing a fatty acid hydroperoxide, which later on acts as an important signaling molecule. For the case of 12R-LOX, 12R-hydroperoxyeicosatetraenoic acid (12R-HPETE) is produced when adding an oxygen molecule. 12R-HPETE is then transformed into a signaling molecule important for lipid formation in the epidermis, which serves a necessary role for preventing skin dehydration [1]. | ||
| − | The gene codes for one of the enzymes used for [https://2024.igem.wiki/linkoping/ iGEM24_Linkoping] substitution therapy for | + | The gene codes for one of the enzymes used for [https://2024.igem.wiki/linkoping/ iGEM24_Linkoping] substitution therapy for ichthyosis. |
===Sequence=== | ===Sequence=== | ||
Revision as of 13:28, 29 September 2024
ALOX12B
Usage and Biology
ALOX12B is a gene found on human chromosome 17 and encodes for the enzyme 12R-LOX. The enzyme is an example of an arachidonate lipoxygenase, which is a family of enzymes that has arachidonic acid as a substrate. The enzymes will add an oxygen molecule to the substrate producing a fatty acid hydroperoxide, which later on acts as an important signaling molecule. For the case of 12R-LOX, 12R-hydroperoxyeicosatetraenoic acid (12R-HPETE) is produced when adding an oxygen molecule. 12R-HPETE is then transformed into a signaling molecule important for lipid formation in the epidermis, which serves a necessary role for preventing skin dehydration [1].
The gene codes for one of the enzymes used for iGEM24_Linkoping substitution therapy for ichthyosis.
Sequence
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1789
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 722
Illegal BamHI site found at 2070 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1747
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 454
References
[1] Krieg P, Fürstenberger G. The role of lipoxygenases in epidermis. Biochim Biophys Acta. 2014 Mar;1841(3):390-400. doi: 10.1016/j.bbalip.2013.08.005. Epub 2013 Aug 16. Erratum in: Biochim Biophys Acta. 2014 Dec;1841(12):1767. PMID: 23954555.