Difference between revisions of "Part:BBa K5382160"

Revision as of 13:22, 29 September 2024

InaK-linker-4z domain_protein domain that have the ability to bind to lgG

InaK is a kind of ice nucleoprotein of Pseudomonas syringae KCTC1832. Ice nucleoprotein is a kind of protein that can promote the formation of ice crystals in organisms. Its N-terminal domain can be tightly anchored to the surface of Escherichia coli cells as an anchor moda, so as to enable surface display.
i Linker here is a short peptide used to connect the Inak and z domain.
z domain refers to a synthetic protein domain that has the ability to bind to the Fc region of immunoglobulin G (IgG). In our project, z domains are used to construct extracellular vesicles (EVs) that can display antibodies, so that these EVs can bind specific antibodies through the z domain on their surface, so as to achieve targeted delivery to specific cells or tissues [1]. The 4z domain, a tetramer of the z domain, has been shown in the literature to be equally capable of constructing EVs displaying antibodies, and has similar binding activity to the z domain.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 2299
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 722
Illegal NgoMIV site found at 962
Illegal NgoMIV site found at 1130
Illegal AgeI site found at 517
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 692
Illegal BsaI.rc site found at 1182
Illegal SapI.rc site found at 326
Illegal SapI.rc site found at 1951

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K5382160 short</partinfo>
-InaK is a kind of ice nucleoprotein of Pseudomonas syringae KCTC1832. Ice nucleoprotein is a kind of protein that can promote the formation of ice crystals in organisms. Its N-terminal domain can be tightly anchored to the surface of Escherichia coli</i> cells as an anchor moda, so as to enable surface display.<br>i
+InaK is a kind of ice nucleoprotein of Pseudomonas syringae KCTC1832. Ice nucleoprotein is a kind of protein that can promote the formation of ice crystals in organisms. Its N-terminal domain can be tightly anchored to the surface of Escherichia coli cells as an anchor moda, so as to enable surface display.<br>i
 Linker here is a short peptide used to connect the Inak and z domain.<br>
 z domain refers to a synthetic protein domain that has the ability to bind to the Fc region of immunoglobulin G (IgG). In our project, z domains are used to construct extracellular vesicles (EVs) that can display antibodies, so that these EVs can bind specific antibodies through the z domain on their surface, so as to achieve targeted delivery to specific cells or tissues [1].