Difference between revisions of "Part:BBa K5108004:Design"

 
Line 1: Line 1:
  
 
__NOTOC__
 
__NOTOC__
−
<partinfo>BBa_K5108004 short</partinfo>
+
<partinfo>BBa_K5108008 short</partinfo>
  
−
<partinfo>BBa_K5108004 SequenceAndFeatures</partinfo>
+
<partinfo>BBa_K5108008 SequenceAndFeatures</partinfo>
  
  
−
===Design Notes===
+
<html>
−
ZZZZZZZZZZZZZZ
+
<h2 style="color: blue;">Sources</h2>
 +
<p>The codon optimized nucleotidic sequence from <i>Pseudomonas putida</i> adapted to <i>Pseudomonas putida</i> is obtained thanks to the Codon Optimization Tool from Integrated DNA Technologies <a href="https://eu.idtdna.com/pages/tools/codon-optimization-tool?returnurl=%2FCodonOpt" target="blank">codon optimizer tool.</a>. This step permits to lower complexity and minimize secondary structures. The Part was obtained through IDT gBlock synthesis</p>
  
−
 
+
<h2 style="color: blue;">References</h2>
−
 
+
<p><ul><i>
−
===Source===
+
<li>Blumer, C., Heeb, S., Pessi, G., & Haas, D. (1999). Global GacA-steered control of cyanide and exoprotease production in Pseudomonas fluorescens involves specific ribosome binding sites. Proceedings Of The National Academy Of Sciences, 96(24), 14073‑14078. https://doi.org/10.1073/pnas.96.24.14073</li>
−
 
+
<li>Blumer, C., Heeb, S., Pessi, G., & Haas, D. (1999). Global GacA-steered control of cyanide and exoprotease production in Pseudomonas fluorescens involves specific ribosome binding sites. Proceedings Of The National Academy Of Sciences, 96(24), 14073‑14078. https://doi.org/10.1073/pnas.96.24.14073</li>
−
YYYYYYYYYYYYy
+
</i></ul></p>
−
 
+
−
===References===
+

Revision as of 12:58, 29 September 2024


hcnA RBS from Pseudomonas aeruginosa (PAO1)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Sources

The codon optimized nucleotidic sequence from Pseudomonas putida adapted to Pseudomonas putida is obtained thanks to the Codon Optimization Tool from Integrated DNA Technologies codon optimizer tool.. This step permits to lower complexity and minimize secondary structures. The Part was obtained through IDT gBlock synthesis

References

  • Blumer, C., Heeb, S., Pessi, G., & Haas, D. (1999). Global GacA-steered control of cyanide and exoprotease production in Pseudomonas fluorescens involves specific ribosome binding sites. Proceedings Of The National Academy Of Sciences, 96(24), 14073‑14078. https://doi.org/10.1073/pnas.96.24.14073
  • Blumer, C., Heeb, S., Pessi, G., & Haas, D. (1999). Global GacA-steered control of cyanide and exoprotease production in Pseudomonas fluorescens involves specific ribosome binding sites. Proceedings Of The National Academy Of Sciences, 96(24), 14073‑14078. https://doi.org/10.1073/pnas.96.24.14073