Difference between revisions of "Part:BBa K5108006"
Yohannquivet (Talk | contribs) |
Yohannquivet (Talk | contribs) |
||
Line 21: | Line 21: | ||
<h2 style="color: blue;"> <b>Usage and Biology</b></h2> | <h2 style="color: blue;"> <b>Usage and Biology</b></h2> | ||
− | <p>< | + | <p>Ribosome Binding Site of gene <i>aprA</i> of <i>P. fluorescens</i>. It was used for protein translation in <i>P. fluorescens</i> from [1]. |
− | + | This RBS was synthesized in an operon with two genes and another RBS and cloned into a plasmid. After transformation in <i>P. fluorescens</i>, our team had successful protein expression with this RBS. Check out composite part <a href="https://parts.igem.org/Part:BBa_K5108009" target="blank">BBa_K5108009</a>for more information. | |
</p> | </p> | ||
− | |||
<br> | <br> | ||
<h2 style="color: blue;"><b>References</b></h2> | <h2 style="color: blue;"><b>References</b></h2> | ||
Line 30: | Line 29: | ||
<ol> | <ol> | ||
<i> | <i> | ||
− | <li> | + | <li>Blumer, C., Heeb, S., Pessi, G., & Haas, D. (1999). Global GacA-steered control of cyanide and exoprotease production in Pseudomonas fluorescens involves specific ribosome binding sites. Proceedings Of The National Academy Of Sciences, 96(24), 14073‑14078. https://doi.org/10.1073/pnas.96.24.14073</li> |
− | + | ||
</i> | </i> | ||
</ol> | </ol> | ||
</html> | </html> |
Revision as of 12:53, 29 September 2024
aprA RBS from Pseudomonas protegens (CHA0)
- Contents
- Usage and Biology
- References
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
Ribosome Binding Site of gene aprA of P. fluorescens. It was used for protein translation in P. fluorescens from [1]. This RBS was synthesized in an operon with two genes and another RBS and cloned into a plasmid. After transformation in P. fluorescens, our team had successful protein expression with this RBS. Check out composite part BBa_K5108009for more information.
References
- Blumer, C., Heeb, S., Pessi, G., & Haas, D. (1999). Global GacA-steered control of cyanide and exoprotease production in Pseudomonas fluorescens involves specific ribosome binding sites. Proceedings Of The National Academy Of Sciences, 96(24), 14073‑14078. https://doi.org/10.1073/pnas.96.24.14073