Difference between revisions of "Part:BBa K5117000"
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===Usage and Biology===
 
===Usage and Biology===
  
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===Design===
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 12:35, 29 September 2024


BsRBS

This part consists of the ribosome binding site of Bacillus subtilis (already described by Vellanoweth & Rabinowitz 1992) followed by a 7 bp spacer.

BsRBS only served for design purposes of the TU Dresden iGEM 2024 Team. This part was neither built nor characterized in the laboratory.


Target organism: Bacillus subtilis

Main purpose of use:: Gene expression and protein production using the host Bacillus subtilis


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Vellanoweth R. L. & Rabinowitz J. C. (1992): The influence of ribosome‐binding‐site elements on translational efficiency in Bacillus subtilis and Escherichia coli in vivo. Molecular microbiology 6(9), 1105-1114. https://doi.org/10.1111/j.1365-2958.1992.tb01548.x