Difference between revisions of "Part:BBa K196014"

Latest revision as of 22:00, 21 October 2009

Final GluColi device.

This is the most important biobrick we constructed in order to test the glue production by E. coli. HfsG [1] and hfsH [2] genes come from Caulobacter crescentus. C.crescentus is an aquatic, Gram-negative bacterium that divides asymmetrically. Besides the bacterium is able to synthesize a strong glue. This glue is mainly composed of polysaccharide. Different proteins are required in the holdfast synthesis, export and attachment in C.crescentus (for more details, please refer to the "introduction" section). In our project, we wanted to implement the glue production in Escherichia coli. As E. coli possesses homolog proteins, we decided to only insert the hfsG and hfsH genes in the plasmid. RFP has been added as a reporter gene.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 2263
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1273
Illegal AgeI site found at 636
Illegal AgeI site found at 748
Illegal AgeI site found at 1172
Illegal AgeI site found at 1657
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K196014 short</partinfo>
-This is the part we actually created to test the glue production by ''E. coli''.
+This is the most important biobrick we constructed in order to test the glue production by <i>E. coli</i>. HfsG [https://parts.igem.org/Part:BBa_K196002:Design] and hfsH [https://parts.igem.org/Part:BBa_K196003:Design] genes come from <i>Caulobacter crescentus</i>. <i>C.crescentus</i> is an aquatic, Gram-negative bacterium that divides asymmetrically. Besides the bacterium is able to synthesize a strong glue. This glue is mainly composed of polysaccharide. Different proteins are required in the holdfast synthesis, export and attachment in <i>C.crescentus</i> (for more details, please refer to the "introduction" section). In our project, we wanted to implement the glue production in <i>Escherichia coli</i>. As <i>E. coli</i> possesses homolog proteins, we decided to only insert the hfsG and hfsH genes in the plasmid. RFP has been added as a reporter gene.
-HfsG and HfsH come from ''Caulobacter crescentus''. ''Caulobacter crescentus'' is an aquatic, Gram-negative bacterium that divides asymmetrically and is able to synthetize a strong glue. This glue is mainly made of a polysaccharide. There are different proteins needed to synthetize, export and attach it to the stalk of ''C. crescentus''. To see the hole system, please see our wiki page [1]. In our project, we would like this glue to be produced by Escherichia coli. As ''E. coli'' does have similar genes for many of these proteins, but not for HfsG and HfsH, we decided to create a plasmid including only the genes coding for these two proteins. HfsG is a glycosyltransferase and HfsH is a carbohydrate esterase.
-RFP has been added as a reporter.
 <!-- Add more about the biology of this part here
 ===Usage and Biology===