Difference between revisions of "Part:BBa K187155"

 
 
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<partinfo>BBa_K187155 short</partinfo>
 
<partinfo>BBa_K187155 short</partinfo>
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trpC is a bifunction enzyme that serves as both indole-3-glycerol phosphate synthase and phosphoribosylanthranilate isomerase. TrpC performs the third and fourth steps of tryptophan synthesis.
  
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This primer is for a gene deemed to be essential for a minimal E.coli genome by Team Biobytes. This primer is designed to amplify only the open reading frame, and produce ends that can be digested by restriction enzymes for insertion into an XbaI/PstI digested plasmid. These restriction sites were designed to be complementary to the restrictions sites found in pAB and pBA (PstI and XbaI). Due to the use of restriction enzymes, it was important to check each gene sequence for those same restriction sites. Genes which contained at least one PstI site, used the NsiI enzyme to be cloned into our standard plasmids (it produces the same overhang as PstI). Similarly if NsiI and PstI were in the gene sequence, SbfI was used. If all three of these sites were present in a gene sequence, other enzymes which produce the same overhang could be used. Genes which contained XbaI could use AvrII, NheI, as well as a multitude of others.
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This gene is present in plasmid pAB (part BBa_K187000), which is one of two universal plasmids for the BioBytes assembly method. For details of the BioBytes method, see RFC 47
  
 
<!-- Add more about the biology of this part here
 
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Latest revision as of 21:59, 21 October 2009

trpC in pAB trpC is a bifunction enzyme that serves as both indole-3-glycerol phosphate synthase and phosphoribosylanthranilate isomerase. TrpC performs the third and fourth steps of tryptophan synthesis.

This gene is present in plasmid pAB (part BBa_K187000), which is one of two universal plasmids for the BioBytes assembly method. For details of the BioBytes method, see RFC 47

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 10
    Illegal PstI site found at 1375
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1375
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 380
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 10
    Illegal PstI site found at 1375
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 10
    Illegal PstI site found at 1375
  • 1000
    COMPATIBLE WITH RFC[1000]