Difference between revisions of "Part:BBa K5387000:Design"
| Line 2: | Line 2: | ||
The sequence has been optimized for expression in ''E. coli''. | The sequence has been optimized for expression in ''E. coli''. | ||
| − | Primers for cloning into | + | The vector used was pET-28a(+), which His-ag we utilized when expressing our protein. |
| + | |||
| + | Primers for cloning into pET-28a(+) between restriction sites NdeI and EcoRI: | ||
: fwd: 5'-GCGCCATATGGCAACATATAAAGTTAGGG-3' | : fwd: 5'-GCGCCATATGGCAACATATAAAGTTAGGG-3' | ||
: rev: 5'-GCGCGAATTCTTAGATGCTGATGG-3' | : rev: 5'-GCGCGAATTCTTAGATGCTGATGG-3' | ||
Latest revision as of 10:31, 29 September 2024
Design Notes
The sequence has been optimized for expression in E. coli.
The vector used was pET-28a(+), which His-ag we utilized when expressing our protein.
Primers for cloning into pET-28a(+) between restriction sites NdeI and EcoRI:
- fwd: 5'-GCGCCATATGGCAACATATAAAGTTAGGG-3'
- rev: 5'-GCGCGAATTCTTAGATGCTGATGG-3'