Difference between revisions of "Part:BBa K5507008:Experience"

 
 
Line 19: Line 19:
 
<!-- End of the user review template -->
 
<!-- End of the user review template -->
 
<!-- DON'T DELETE --><partinfo>BBa_K5507008 EndReviews</partinfo>
 
<!-- DON'T DELETE --><partinfo>BBa_K5507008 EndReviews</partinfo>
 +
 +
a. Construction of BBa_K5507008
 +
For the overexpression of the pgi gene from E. coli, the sacB locus, which encodes levansucrase, was selected as a homologous recombination site because knocking out this gene has no metabolic effects under glucose conditions. First, the kana expression cassette will replace the sacB gene locus as a selectable marker. Then, pgi will replace the kana gene, resulting in the loss of kana resistance, confirming successful gene insertion.
 +
 +
<html>
 +
<body>
 +
<figure>
 +
<div class = "center">
 +
<center>< img src = "https://static.igem.wiki/teams/5507/parts-figures/hw-figure-1.png" style = "width:500px"></center>
 +
</div>
 +
<figcaption><center> Figure 1. Gene map of BBa_K5507008</center></figcaption>
 +
</figure>
 +
</body>
 +
</html>

Latest revision as of 09:04, 29 September 2024


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K5507008

User Reviews

UNIQ7e8b7946ccded001-partinfo-00000000-QINU UNIQ7e8b7946ccded001-partinfo-00000001-QINU

a. Construction of BBa_K5507008 For the overexpression of the pgi gene from E. coli, the sacB locus, which encodes levansucrase, was selected as a homologous recombination site because knocking out this gene has no metabolic effects under glucose conditions. First, the kana expression cassette will replace the sacB gene locus as a selectable marker. Then, pgi will replace the kana gene, resulting in the loss of kana resistance, confirming successful gene insertion.

< img src = "https://static.igem.wiki/teams/5507/parts-figures/hw-figure-1.png" style = "width:500px">
Figure 1. Gene map of BBa_K5507008