Difference between revisions of "Part:BBa K180017"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K180017 short</partinfo>
 
<partinfo>BBa_K180017 short</partinfo>
  
 
Composite part, consisting of BBa_K180018 and BBa_K180019, but with BBa_K180018 reading along the reverse strand, and BBa_K180019 reading along the forward strand.
 
Composite part, consisting of BBa_K180018 and BBa_K180019, but with BBa_K180018 reading along the reverse strand, and BBa_K180019 reading along the forward strand.
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BBa_K180018 reads along reverse strand, and BBa_K180019 reads along the forward strand to ensure the constitutive promoter BBa_J23119 (present in BBa_K180018) has no effect over mBanana or LuxI expression in BBa_K180019.
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mBanana and LuxI are generated under tac pR regulation, whilst LasR is produced constitutively.
  
 
Forms the 'scissors' primary plasmid in Team:Southampton's rock-paper-scissors project design.
 
Forms the 'scissors' primary plasmid in Team:Southampton's rock-paper-scissors project design.
  
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<!-- Add more about the biology of this part here
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===Usage and Biology===
 
===Usage and Biology===
  
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This part has been specified as a basic BioBrick, due to some of the component parts reading in reverse (unable to specify using the component BioBrick format).
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To function as the 'Scissors' in the rock-paper-scissors design by Team:Southampton, the part has to be used in conjunction with K180020, and pairs K180010 & K180013, K180014 & K180016.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K180017 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K180017 SequenceAndFeatures</partinfo>

Latest revision as of 21:28, 21 October 2009

Rock-paper-scissors - Scissors primary plasmid

Composite part, consisting of BBa_K180018 and BBa_K180019, but with BBa_K180018 reading along the reverse strand, and BBa_K180019 reading along the forward strand.

BBa_K180018 reads along reverse strand, and BBa_K180019 reads along the forward strand to ensure the constitutive promoter BBa_J23119 (present in BBa_K180018) has no effect over mBanana or LuxI expression in BBa_K180019.

mBanana and LuxI are generated under tac pR regulation, whilst LasR is produced constitutively.

Forms the 'scissors' primary plasmid in Team:Southampton's rock-paper-scissors project design.


Usage and Biology

This part has been specified as a basic BioBrick, due to some of the component parts reading in reverse (unable to specify using the component BioBrick format).

To function as the 'Scissors' in the rock-paper-scissors design by Team:Southampton, the part has to be used in conjunction with K180020, and pairs K180010 & K180013, K180014 & K180016.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2754
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 887
    Illegal NheI site found at 910
    Illegal PstI site found at 2754
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2754
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2754
    Illegal NgoMIV site found at 534
    Illegal NgoMIV site found at 3000
    Illegal AgeI site found at 337
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2943