Difference between revisions of "Part:BBa K5378012"

 
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__NOTOC__
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<partinfo>BBa_K5378012 short</partinfo>
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<partinfo>BBa_K5378012 SequenceAndFeatures</partinfo>
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===Design Notes===
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To create therapeutic curli fibers, we plan to attach TFF3 (Trefoil Factor 3) to the curli fibers naturally secreted by E. coli to reduce TFF3's adhesion to the intestinal mucus layer. This will maximize TFF3's ability to repair the intestinal barrier and reduce inflammation. Effective prevention and treatment of abdominal infections in liver disease patients, addressing dysbiosis, and specifically repairing common intestinal issues will enhance the safety and efficacy of the engineered bacterium.
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For this purpose, we referred to a study published in Nature Communications And the PATCH system was used for plasmid design. We first linked the gene fragments responsible for expressing curli fibers to the PBbB8k plasmid, then introduced a 6xHis-tagged linker to connect curli fibers with TFF3, and finally incorporated the TFF3 gene fragment. This configuration allows EcN to secrete and self-assemble curli fibers, linkers, and TFF3 upon reaching the intestine, forming an active domain layer on the intestinal surface. This promotes epithelial cell migration, reduces inflammatory factor levels, supports intestinal barrier repair, and alleviates hepatic encephalopathy complications.
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===References===
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[1]Taupin, D. & Podolsky, D. K. Trefoil factors: initiators of mucosal healing.Nat. Rev. Mol. Cell Biol. 4, 721 (2003).
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[2]Aamann, L., Vestergaard, E. M. & Grønbæk, H. Trefoil factors in inflammatory bowel disease. World J. Gastroenterology: Wjg. 20, 3223–3230(2014).

Revision as of 07:16, 29 September 2024


TFF3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To create therapeutic curli fibers, we plan to attach TFF3 (Trefoil Factor 3) to the curli fibers naturally secreted by E. coli to reduce TFF3's adhesion to the intestinal mucus layer. This will maximize TFF3's ability to repair the intestinal barrier and reduce inflammation. Effective prevention and treatment of abdominal infections in liver disease patients, addressing dysbiosis, and specifically repairing common intestinal issues will enhance the safety and efficacy of the engineered bacterium. For this purpose, we referred to a study published in Nature Communications And the PATCH system was used for plasmid design. We first linked the gene fragments responsible for expressing curli fibers to the PBbB8k plasmid, then introduced a 6xHis-tagged linker to connect curli fibers with TFF3, and finally incorporated the TFF3 gene fragment. This configuration allows EcN to secrete and self-assemble curli fibers, linkers, and TFF3 upon reaching the intestine, forming an active domain layer on the intestinal surface. This promotes epithelial cell migration, reduces inflammatory factor levels, supports intestinal barrier repair, and alleviates hepatic encephalopathy complications.

References

[1]Taupin, D. & Podolsky, D. K. Trefoil factors: initiators of mucosal healing.Nat. Rev. Mol. Cell Biol. 4, 721 (2003).

[2]Aamann, L., Vestergaard, E. M. & Grønbæk, H. Trefoil factors in inflammatory bowel disease. World J. Gastroenterology: Wjg. 20, 3223–3230(2014).