Difference between revisions of "Part:BBa K5034213:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | We synthesized the fragment using chemical synthesis method. | + | We synthesized the fragment using a chemical synthesis method, with the aim of expressing the protein in Shewanella and regulating cellular phosphorus metabolism and electron transfer. |
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===Source=== | ===Source=== |
Revision as of 07:11, 29 September 2024
PolyP <->Pi
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We synthesized the fragment using a chemical synthesis method, with the aim of expressing the protein in Shewanella and regulating cellular phosphorus metabolism and electron transfer.
Source
Polyphosphate kinase 1(PPK1) from Citrobacter freundii. NBCI reference sequence: NZ_LR890181.1:c1367205-1365139
References
Wang, X., Wang, X., Hui, K., Wei, W., Zhang, W., Miao, A., . . . Yang, L. (2018). Highly Effective Polyphosphate Synthesis, Phosphate Removal, and Concentration Using Engineered Environmental Bacteria Based on a Simple Solo Medium-Copy Plasmid Strategy. Environmental Science & Technology, 52(1), 214-222. doi:10.1021/acs.est.7b04532