Difference between revisions of "Part:BBa K292004:Design"

 
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<partinfo>BBa_K292004 short</partinfo>
 
<partinfo>BBa_K292004 short</partinfo>
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When we design the bio-brick it was impossible to obtain a functional bio-brick by using the plasmid SB1AK3. But it's worked with a pSB1A1.
 
When we design the bio-brick it was impossible to obtain a functional bio-brick by using the plasmid SB1AK3. But it's worked with a pSB1A1.
  
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[[Part:BBa_P1003|BBa_P1003]] is an kanamycin antibiotic resistance cassette. Several silent mutations have been introduced to eliminate useful restriction sites and rare codons. However, we inadvertently introduced a new BsmBI recognition site in BBa_P1003 (kanamycin antibiotic resistance marker) during the course of codon shuffling. <br>
  
 
===Source===
 
===Source===

Revision as of 21:17, 21 October 2009

Double terminator + Kanamycin resistance cassette + Double ter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When we design the bio-brick it was impossible to obtain a functional bio-brick by using the plasmid SB1AK3. But it's worked with a pSB1A1.

BBa_P1003 is an kanamycin antibiotic resistance cassette. Several silent mutations have been introduced to eliminate useful restriction sites and rare codons. However, we inadvertently introduced a new BsmBI recognition site in BBa_P1003 (kanamycin antibiotic resistance marker) during the course of codon shuffling.

Source

Part:BBa_B0014, Designed by Reshma Shetty Group: Registry (2003-07-16) Part:BBa_P1003, Designed by Reshma Shetty Group: Knight Lab, MIT (2006-01-23)

References