Difference between revisions of "Part:BBa K5241004"

Revision as of 06:02, 29 September 2024

Mel

Short Description: The ADE2 gene is combined with the yeast gal1 promoter, the enhanced green fluorescent protein gene egfp, and the CYC1 terminator.

genetic map：

Source:Pseudomonas maltophilia

Description:

The role of the mel gene in brewing yeast is mainly as a reporter gene. The tyrosinase enzyme it encodes can catalyze the conversion of tyrosine to dopamine, which is then further converted into melanin. This produced melanin is non-toxic and harmless, allowing for the direct observation of a black phenotype on the culture medium without the need to add color-developing compounds or use special instruments.

Result

We successfully expressed the mel gene,the melanin expressed by the mel gene in brewing yeast appears red, possibly because a larger amount of pheomelanin is produced.

Reference documentation

[1] Ruohola, H., Liljeström, P. L., Torkkeli, T., Kopu, H., Lehtinen, P., Kalkkinen, N., & Korhola, M. (1986). Expression and regulation of the yeast MEL1 gene. FEMS Microbiology Letters, 34(2), 179–185.

[2] Naumov, G., Naumova, E., Turakainen, H., Suominen, P., & Korhola, M. (1990). A new family of polymorphic genes in Saccharomyces cerevisiae: alpha-galactosidase genes MEL1-MEL7. Molecular Genetics and Genomics, 224(1), 119–128.

Sequence and Features