Difference between revisions of "Part:BBa K5136059"

Revision as of 05:53, 29 September 2024

rhlB
Transferase activity, transferring hexosyl groups.

Biology

The rhamnosyltransferase (RhlB) comes from Pseudomonas aeruginosa, is capable of catalyzing the reaction between HAA and dTDP-l-rhamnose to form mono-rhamnolipid. (1) The hydropathy plot of the RhlB suggested that the protein is membrane-anchored via its N-terminal, crossing the membrane and exposing its domains on both sides of the membrane.

Usage

We used BBa_K081005 to construct the expression system and obtained the composite BBa_K5136232, which is assembled on the expression vector pSB1C3 by standard assembly. The constructed plasmid was transformed into E. coli DH5α, then the positive transformants were selected by chloramphenicol and confirmed by colony PCR and sequencing.

Reference

1. Chong, H.; Li, Q., Microbial production of rhamnolipids: opportunities, challenges and strategies. Microb Cell Fact 2017, 16 (1), 137.
2. Ochsner, U. A.; Fiechter, A.; Reiser, J., Isolation, characterization, and expression in Escherichia coli of the Pseudomonas aeruginosa rhlAB genes encoding a rhamnosyltransferase involved in rhamnolipid biosurfactant synthesis. J Biol Chem 1994, 269 (31), 19787-19795.

Sequence and Features