Difference between revisions of "Part:BBa K5291027:Design"

(→‎Design Notes)
(→‎References)
 
Line 14: Line 14:
  
 
===References===
 
===References===
 +
Oka SI, Titus AS, Zablocki D, Sadoshima J. Molecular properties and regulation of NAD+ kinase (NADK). Redox Biol. 2023 Feb;59:102561. doi: 10.1016/j.redox.2022.102561. Epub 2022 Dec 5. PMID: 36512915; PMCID: PMC9763689.

Latest revision as of 05:06, 29 September 2024


nadK: nicotinamide adenine dinucleotide kinase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We mainly consider the role of nadK in changing intracellular NAD+ and NADP+ levels. Given the NADPH requirement of the engineered bacteria, we decided to introduce NADK encoded by nadK. NADK can phosphorylate NAD and produce NADP(H). NADK is the only known mechanism by which NADP(H) is produced de novo.

Source

Rhodopseudomonas palustris strain RCB100

References

Oka SI, Titus AS, Zablocki D, Sadoshima J. Molecular properties and regulation of NAD+ kinase (NADK). Redox Biol. 2023 Feb;59:102561. doi: 10.1016/j.redox.2022.102561. Epub 2022 Dec 5. PMID: 36512915; PMCID: PMC9763689.