Difference between revisions of "Part:BBa K5269023"

Revision as of 03:47, 29 September 2024

SpyTag-Mgfp-5-His-Tag and BsmB I cut site(for GGA)

It contains SD-sequences(BBa_K5269034), SpyTag, Mgfp-5 (BBa_5269013) and His-Tag sequences. In addition, the BsmB I cleavage sites at both ends of the sequence allow for Golden Gate Assembly to the Golden Gate Assembly Site (BBa_K5269020) and T7 promoter-shRNA-T7 terminator-Golden Gate Assembly Site(BBa_K5269025). The SpyCatcher-SpyTag system works when used with Lpp-ompA-SpyCatcher(BBa_K5269026) or INPNC-SpyCatcher(BBa_K5269027). Therefore, we expect the adhesion protein Mgfp-5 to be stable on the Membrane Vesicles, which will facilitate the retention of our shRNA-encapsulated membrane vesicles on the plant surface.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 381
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K5269023 short</partinfo>
-It contains SD sequences(BBa_K5269034), SpyTag (BBa_K5269017), Mgfp-5 (BBa_5269013) and 6xHis sequences. In addition, the BsmB I cleavage sites at both ends of the sequence allow for Golden Gate Assembly to the Golden Gate Assembly Site (BBa_K5269020) and T7 promoter-shRNA-T7 terminator-Golden Gate Assembly Site(BBa_K5269025). The SpyTag/SpyCatcher system works when used with Lpp-ompA-SpyCatcher(BBa_K5269026) or INPNC-SpyCatcher(BBa_K5269027). Therefore, we expect the adhesion protein Mgfp-5 to be stable on the membrane vesicles, which will facilitate the retention of our shRNA-encapsulated membrane vesicles on the plant surface.
+It contains SD-sequences(BBa_K5269034), SpyTag, Mgfp-5 (BBa_5269013) and His-Tag sequences. In addition, the BsmB I cleavage sites at both ends of the sequence allow for Golden Gate Assembly to the Golden Gate Assembly Site (BBa_K5269020) and T7 promoter-shRNA-T7 terminator-Golden Gate Assembly Site(BBa_K5269025). The SpyCatcher-SpyTag system works when used with Lpp-ompA-SpyCatcher(BBa_K5269026) or INPNC-SpyCatcher(BBa_K5269027). Therefore, we expect the adhesion protein Mgfp-5 to be stable on the Membrane Vesicles, which will facilitate the retention of our shRNA-encapsulated membrane vesicles on the plant surface.
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