Difference between revisions of "Part:BBa K5115053"
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===Introduction===
 
===Introduction===
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This composite part combines all the subunit, except hoxU, of the hydrogenase in our ribozyme-assisted polycistronic co-expression system：pRAP. To learn more about the hydrogenase, please check [https://parts.igem.org/Part:BBa_K51150020 BBa_K51150020(hox and hyp operon)]. To get more information about pRAP, please check [https://2022.igem.wiki/fudan/parts part wiki of 2022 Fudan iGEM ].
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This composite part combines all the subunit, except hoxU, of the hydrogenase in our ribozyme-assisted polycistronic co-expression system：pRAP. To learn more about the hydrogenase, please check [https://parts.igem.org/Part:BBa_K5115020 BBa_K5115020(hox and hyp operon)]. To get more information about pRAP, please check [https://2022.igem.wiki/fudan/parts part wiki of 2022 Fudan iGEM ].
  
  

Revision as of 03:40, 29 September 2024


ribozyme connected hox and hyp, without hoxU

contributed by Fudan iGEM 2023

Introduction

This composite part combines all the subunit, except hoxU, of the hydrogenase in our ribozyme-assisted polycistronic co-expression system：pRAP. To learn more about the hydrogenase, please check BBa_K5115020(hox and hyp operon). To get more information about pRAP, please check part wiki of 2022 Fudan iGEM .


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 654
    Illegal BglII site found at 1472
    Illegal BglII site found at 1765
    Illegal BamHI site found at 2059
    Illegal XhoI site found at 99
    Illegal XhoI site found at 2266
    Illegal XhoI site found at 4505
    Illegal XhoI site found at 6334
    Illegal XhoI site found at 7496
    Illegal XhoI site found at 9335
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 533
    Illegal NgoMIV site found at 1071
    Illegal NgoMIV site found at 1281
    Illegal NgoMIV site found at 1593
    Illegal NgoMIV site found at 2359
    Illegal NgoMIV site found at 3039
    Illegal NgoMIV site found at 5646
    Illegal AgeI site found at 6821
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4159
    Illegal BsaI.rc site found at 702
    Illegal BsaI.rc site found at 1206


References