Difference between revisions of "Part:BBa K5269007"
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<partinfo>BBa_K5269007 short</partinfo> | <partinfo>BBa_K5269007 short</partinfo> | ||
− | This is a reverse primer used for PCR of the | + | This is a reverse primer used for PCR of the Surface displayed protein-SpyTag complex (BBa_K5269021-BBa_K5269024). This primer is also used for Golden Gate Assembly (GGA) to pBluescript II SK(-) ,which has Golden Gate Assembly Site (BBa_K5269020). This primer is used for GGA of two or more Surface displayed protein-SpyTag complexes, the second of which is closest to the L3S2P21 terminator. |
− | This primer is used for GGA of two or more | + | |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Latest revision as of 03:30, 29 September 2024
Surface displayed protein reverse primer 3
This is a reverse primer used for PCR of the Surface displayed protein-SpyTag complex (BBa_K5269021-BBa_K5269024). This primer is also used for Golden Gate Assembly (GGA) to pBluescript II SK(-) ,which has Golden Gate Assembly Site (BBa_K5269020). This primer is used for GGA of two or more Surface displayed protein-SpyTag complexes, the second of which is closest to the L3S2P21 terminator.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]