Difference between revisions of "Part:BBa K5269006"

 
 
Line 3: Line 3:
 
<partinfo>BBa_K5269006 short</partinfo>
 
<partinfo>BBa_K5269006 short</partinfo>
  
−
This is a reverse primer used for PCR of the surface-presenting protein-SpyTag complex (BBa_K5269021-BBa_K5269024). This primer is also used for Golden Gate Assembly (GGA) to pBluescript II SK(-)  which has Golden Gate Assembly Site (BBa_K5269020).  
+
This is a reverse primer used for PCR of the Surface displayed protein-SpyTag complex (BBa_K5269021-BBa_K5269024). This primer is also used for Golden Gate Assembly (GGA) to pBluescript II SK(-)  which has Golden Gate Assembly Site (BBa_K5269020). This primer is used for GGA of a single Surface displayed protein-SpyTag complex, which is closest to the L3S2P21 terminator.
−
This primer is used for GGA of a single surface-presenting protein-SpyTag complex, which is closest to the L3S2P21 terminator.
+
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 03:27, 29 September 2024


Surface displayed protein reverse primer 2

This is a reverse primer used for PCR of the Surface displayed protein-SpyTag complex (BBa_K5269021-BBa_K5269024). This primer is also used for Golden Gate Assembly (GGA) to pBluescript II SK(-) which has Golden Gate Assembly Site (BBa_K5269020). This primer is used for GGA of a single Surface displayed protein-SpyTag complex, which is closest to the L3S2P21 terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]