Difference between revisions of "Part:BBa K243017:Design"

(→‎Design Notes)
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The choice of the linker, lipocalin tag and purification tag allows us several different combinations.
 
The choice of the linker, lipocalin tag and purification tag allows us several different combinations.
 
[https://static.igem.org/mediawiki/parts/0/04/Strepdigasplitfoka.txt Commented GenBank file]
 
[https://static.igem.org/mediawiki/parts/0/04/Strepdigasplitfoka.txt Commented GenBank file]
−
The composite part was cloned step by step, we started with the His-Tag fused it with DigA. In the next step we fused behind the DigA the SplitLinker, in the final step we fused Fok_i to the construct. All cloning steps to fuse the single parts were proceed by the [https://parts.igem.org/Assembly_standard_25 RFC 25].
+
The composite part was cloned step by step. We started with the His-Tag and fused it to DigA. In the next step we fused the SplitLinker behind the DigA, in the final step we fused Fok_i to the construct. All cloning steps to fuse the single parts were accomplished according to the [https://parts.igem.org/Assembly_standard_25 RFC 25].
  
 
===Source===
 
===Source===

Revision as of 21:04, 21 October 2009

Strep-DigA-Split Linker-Fok_a


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 278
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1132


Design Notes

The choice of the linker, lipocalin tag and purification tag allows us several different combinations. Commented GenBank file The composite part was cloned step by step. We started with the His-Tag and fused it to DigA. In the next step we fused the SplitLinker behind the DigA, in the final step we fused Fok_i to the construct. All cloning steps to fuse the single parts were accomplished according to the RFC 25.

Source

Combined the parts by serial cloning steps.

References