Difference between revisions of "Part:BBa K5119017"
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<p>This part is a full-length spectinomycin antibiotic resistance promoter originally found in Streptomyces spectabilis and obtained from pBTK403, a broad-host-range bacterial origin plasmid (Leonard et al., 2018). Through our research, we offer a collection of parts (BBa_K5119000 to BBa_K5119087) that enables researchers to assemble their own plasmid that can replicate in both gram-positive species and E. coli, with the added functionality of secreting enzymes capable of degrading gliadin. Explore the entire collection of parts associated with our project on the Parts page of the UT Austin 2024 iGEM Wiki. </p>
  
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<center><b> Figure 1: </b> The UT-Austin 2024 iGEM parts collection. This collection includes 22 constitutive antibiotic resistance promoters (Type 2), 9 secretion tags (Type 3a), 2 reporter proteins and 4 reporter proteins & enzymes (Type 3b), a rpoC terminator (Type 4), and 3 plasmid backbones (Type 56781). Created with Biorender.com."</center>
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<h2>Sequence and Features</h2>
 
<h2>Sequence and Features</h2>
 
<partinfo>BBa_K5119017 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K5119017 SequenceAndFeatures</partinfo>
 
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Revision as of 03:03, 29 September 2024


SpcR FL Promoter

This part is a full-length spectinomycin antibiotic resistance promoter originally found in Streptomyces spectabilis and obtained from pBTK403, a broad-host-range bacterial origin plasmid (Leonard et al., 2018). Through our research, we offer a collection of parts (BBa_K5119000 to BBa_K5119087) that enables researchers to assemble their own plasmid that can replicate in both gram-positive species and E. coli, with the added functionality of secreting enzymes capable of degrading gliadin. Explore the entire collection of parts associated with our project on the Parts page of the UT Austin 2024 iGEM Wiki.

Figure 1: The UT-Austin 2024 iGEM parts collection. This collection includes 22 constitutive antibiotic resistance promoters (Type 2), 9 secretion tags (Type 3a), 2 reporter proteins and 4 reporter proteins & enzymes (Type 3b), a rpoC terminator (Type 4), and 3 plasmid backbones (Type 56781). Created with Biorender.com."

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]