Difference between revisions of "Part:BBa K5241004"

 
Line 4: Line 4:
  
 
The ADE2 gene is combined with the yeast gal1 promoter, the enhanced green fluorescent protein gene egfp, and the CYC1 terminator.
 
The ADE2 gene is combined with the yeast gal1 promoter, the enhanced green fluorescent protein gene egfp, and the CYC1 terminator.
 +
 +
<span style="font-size: 150%;font-weight: bold;">genetic map：</span>
 +
 +
<html>
 +
<figure>
 +
<img style="width: 70%; padding:28px;"src="https://static.igem.wiki/teams/5241/wiki/parts-static/4/bba-k5241004-img01.png" >
 +
</figure>
 +
</html>
 +
 +
<span style="font-weight: bold;">Source:Pseudomonas maltophilia</span>
 +
 +
<span style="font-size: 150%;font-weight: bold;">Description:</span>
 +
 +
The role of the mel gene in brewing yeast is mainly as a reporter gene. The tyrosinase enzyme it encodes can catalyze the conversion of tyrosine to dopamine, which is then further converted into melanin. This produced melanin is non-toxic and harmless, allowing for the direct observation of a black phenotype on the culture medium without the need to add color-developing compounds or use special instruments.
 +
 +
<span style="font-size: 150%;font-weight: bold;">Result</span>
 +
 +
 +
<html>
 +
<figure>
 +
<img style="width: 70%; padding:28px;"src="https://static.igem.wiki/teams/5241/wiki/parts-static/4/bba-k5241004-img02.png" >
 +
</figure>
 +
</html>
 +
 +
We successfully expressed the mel gene,the melanin expressed by the mel gene in brewing yeast appears red, possibly because a larger amount of pheomelanin is produced.
 +
 +
<span style="font-size: 150%;font-weight: bold;">Reference documentation</span>
 +
 +
[1] Ruohola, H., Liljeström, P. L., Torkkeli, T., Kopu, H., Lehtinen, P., Kalkkinen, N., & Korhola, M. (1986). Expression and regulation of the yeast MEL1 gene. FEMS Microbiology Letters, 34(2), 179–185.
 +
 +
[2] Naumov, G., Naumova, E., Turakainen, H., Suominen, P., & Korhola, M. (1990). A new family of polymorphic genes in Saccharomyces cerevisiae: alpha-galactosidase genes MEL1-MEL7. Molecular Genetics and Genomics, 224(1), 119–128.
 +
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 01:21, 29 September 2024


Mel

The ADE2 gene is combined with the yeast gal1 promoter, the enhanced green fluorescent protein gene egfp, and the CYC1 terminator.

genetic map：

Source:Pseudomonas maltophilia

Description:

The role of the mel gene in brewing yeast is mainly as a reporter gene. The tyrosinase enzyme it encodes can catalyze the conversion of tyrosine to dopamine, which is then further converted into melanin. This produced melanin is non-toxic and harmless, allowing for the direct observation of a black phenotype on the culture medium without the need to add color-developing compounds or use special instruments.

Result


We successfully expressed the mel gene,the melanin expressed by the mel gene in brewing yeast appears red, possibly because a larger amount of pheomelanin is produced.

Reference documentation

[1] Ruohola, H., Liljeström, P. L., Torkkeli, T., Kopu, H., Lehtinen, P., Kalkkinen, N., & Korhola, M. (1986). Expression and regulation of the yeast MEL1 gene. FEMS Microbiology Letters, 34(2), 179–185.

[2] Naumov, G., Naumova, E., Turakainen, H., Suominen, P., & Korhola, M. (1990). A new family of polymorphic genes in Saccharomyces cerevisiae: alpha-galactosidase genes MEL1-MEL7. Molecular Genetics and Genomics, 224(1), 119–128.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]