Difference between revisions of "Part:BBa K5490023"
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<partinfo>BBa_K5490023 short</partinfo> | <partinfo>BBa_K5490023 short</partinfo> | ||
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A luciferase reporter containing three target sequences, each 23 nucleotides long and representing specific loci in the WNV genome, has been designed using an algorithmic approach to identify optimal CasRx activity targets. Researchers can modify these sequences to experimentally assess the cleavage efficiency of their own target sequences through a luciferase assay | A luciferase reporter containing three target sequences, each 23 nucleotides long and representing specific loci in the WNV genome, has been designed using an algorithmic approach to identify optimal CasRx activity targets. Researchers can modify these sequences to experimentally assess the cleavage efficiency of their own target sequences through a luciferase assay | ||
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<partinfo>BBa_K5490023 parameters</partinfo> | <partinfo>BBa_K5490023 parameters</partinfo> | ||
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| + | PREPEI NA MPOYN APOTELESMATA | ||
Latest revision as of 23:49, 28 September 2024
LUCIFERASE WITH TARGET SITES IN
It is a basic part of BBa_K5490024
A luciferase reporter containing three target sequences, each 23 nucleotides long and representing specific loci in the WNV genome, has been designed using an algorithmic approach to identify optimal CasRx activity targets. Researchers can modify these sequences to experimentally assess the cleavage efficiency of their own target sequences through a luciferase assay
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1693
Illegal XbaI site found at 1915 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1693
Illegal NotI site found at 1898 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1693
Illegal BamHI site found at 1906 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1693
Illegal XbaI site found at 1915 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1693
Illegal XbaI site found at 1915
Illegal NgoMIV site found at 74
Illegal NgoMIV site found at 1451
Illegal NgoMIV site found at 1472
Illegal NgoMIV site found at 1787
Illegal AgeI site found at 1166 - 1000COMPATIBLE WITH RFC[1000]
PREPEI NA MPOYN APOTELESMATA