Difference between revisions of "Part:BBa K5490036"
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<partinfo>BBa_K5490036 short</partinfo> | <partinfo>BBa_K5490036 short</partinfo> | ||
| − | Is a type of promoter with high specificity to a particular type of transcription factor, in this case, the synthetic NFAT, it achieves this by having various upstream elements of a small DNA sequence that contains the TATA box where polymerase II will bind. "Downstream, it contains a luciferase gene for assessing promoter strength through a luciferase assay. | + | Is a type of promoter with high specificity to a particular type of transcription factor, in this case, the synthetic NFAT (BBa_K5490017), it achieves this by having various upstream elements of a small DNA sequence that contains the TATA box where polymerase II will bind. "Downstream, it contains a luciferase gene for assessing promoter strength through a luciferase assay. |
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<partinfo>BBa_K5490036 parameters</partinfo> | <partinfo>BBa_K5490036 parameters</partinfo> | ||
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| + | Meško M, Lebar T, Dekleva P, Jerala R, Benčina M. Engineering and Rewiring of a Calcium-Dependent Signaling Pathway. ACS Synth Biol. 2020 Aug 21;9(8):2055-2065. doi: 10.1021/acssynbio.0c00133. Epub 2020 Jul 20. PMID: 32643923; PMCID: PMC7467823. | ||
Revision as of 23:37, 28 September 2024
10x[AB]-pMIN-fL
Is a type of promoter with high specificity to a particular type of transcription factor, in this case, the synthetic NFAT (BBa_K5490017), it achieves this by having various upstream elements of a small DNA sequence that contains the TATA box where polymerase II will bind. "Downstream, it contains a luciferase gene for assessing promoter strength through a luciferase assay.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1270
Illegal EcoRI site found at 5571
Illegal XbaI site found at 1457
Illegal XbaI site found at 3239
Illegal SpeI site found at 5325
Illegal PstI site found at 1451
Illegal PstI site found at 4414 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1270
Illegal EcoRI site found at 5571
Illegal SpeI site found at 5325
Illegal PstI site found at 1451
Illegal PstI site found at 4414
Illegal NotI site found at 4393
Illegal NotI site found at 5717
Illegal NotI site found at 5787
Illegal NotI site found at 6082
Illegal NotI site found at 6152 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1270
Illegal EcoRI site found at 5571
Illegal BamHI site found at 1719
Illegal XhoI site found at 6339 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1270
Illegal EcoRI site found at 5571
Illegal XbaI site found at 1457
Illegal XbaI site found at 3239
Illegal SpeI site found at 5325
Illegal PstI site found at 1451
Illegal PstI site found at 4414 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1270
Illegal EcoRI site found at 5571
Illegal XbaI site found at 1457
Illegal XbaI site found at 3239
Illegal SpeI site found at 5325
Illegal PstI site found at 1451
Illegal PstI site found at 4414
Illegal NgoMIV site found at 1028
Illegal NgoMIV site found at 1049
Illegal NgoMIV site found at 1364
Illegal NgoMIV site found at 1472
Illegal NgoMIV site found at 2950
Illegal NgoMIV site found at 2967
Illegal NgoMIV site found at 3099
Illegal AgeI site found at 752
Illegal AgeI site found at 3245
Illegal AgeI site found at 5583
Illegal AgeI site found at 5923
Illegal AgeI site found at 5948
Illegal AgeI site found at 6288 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1740
Illegal SapI site found at 3451
Illegal SapI.rc site found at 934
Meško M, Lebar T, Dekleva P, Jerala R, Benčina M. Engineering and Rewiring of a Calcium-Dependent Signaling Pathway. ACS Synth Biol. 2020 Aug 21;9(8):2055-2065. doi: 10.1021/acssynbio.0c00133. Epub 2020 Jul 20. PMID: 32643923; PMCID: PMC7467823.