Difference between revisions of "Part:BBa K180004"
 
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BBa_K180005 reads along reverse strand, and BBa_K180006 reads along the forward strand to ensure the constitutive promoter BBa_J23119 (present in BBa_K180005) has no effect over LacI or GFP-LVA expression in BBa_K180006.
 
BBa_K180005 reads along reverse strand, and BBa_K180006 reads along the forward strand to ensure the constitutive promoter BBa_J23119 (present in BBa_K180005) has no effect over LacI or GFP-LVA expression in BBa_K180006.
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LacI and GFP-LVA are generated under lux pR regulation, whilst LuxR is constitutively produced.
  
  

Latest revision as of 20:52, 21 October 2009

Game of Life - Primary plasmid

Composite part, consisting of BBa_K180005 and BBa_K180006, but with BBa_K180005 reading along reverse strand, and BBa_K180006 reading along the forward strand.

BBa_K180005 reads along reverse strand, and BBa_K180006 reads along the forward strand to ensure the constitutive promoter BBa_J23119 (present in BBa_K180005) has no effect over LacI or GFP-LVA expression in BBa_K180006.

LacI and GFP-LVA are generated under lux pR regulation, whilst LuxR is constitutively produced.


Usage and Biology

This part has been specified as a basic BioBrick, due to some of the component parts reading in reverse (unable to specify using the component BioBrick format).

To function in the Game of Life design by Team:Southampton, the part has to be used in conjunction with K180007.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 945
    Illegal NheI site found at 968
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2215
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 985
    Illegal BsaI.rc site found at 2891