Difference between revisions of "Part:BBa K180004"

Revision as of 20:49, 21 October 2009

Game of Life - Primary plasmid

Composite part, consisting of BBa_K180005 and BBa_K180006, but with BBa_K180005 reading along reverse strand, and BBa_K180006 reading along the forward strand.

BBa_K180005 reads along reverse strand, and BBa_K180006 reads along the forward strand to ensure the constitutive promoter BBa_J23119 (present in BBa_K180005) has no effect over LacI or GFP-LVA expression in BBa_K180006.

Usage and Biology

This part has been specified as a basic BioBrick, due to some of the component parts reading in reverse (unable to specify using the component BioBrick format).

To function in the Game of Life design by Team:Southampton, the part has to be used in conjunction with K180007.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 945
Illegal NheI site found at 968
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 2215
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 985
Illegal BsaI.rc site found at 2891

@@ Line 3: / Line 3: @@
 Composite part, consisting of BBa_K180005 and BBa_K180006, but with BBa_K180005 reading along reverse strand, and BBa_K180006 reading along the forward strand.
+BBa_K180005 reads along reverse strand, and BBa_K180006 reads along the forward strand to ensure the constitutive promoter BBa_J23119 (present in BBa_K180005) has no effect over LacI or GFP-LVA expression in BBa_K180006.
@@ Line 8: / Line 10: @@
 This part has been specified as a basic BioBrick, due to some of the component parts reading in reverse (unable to specify using the component BioBrick format).
+To function in the Game of Life design by Team:Southampton, the part has to be used in conjunction with K180007.