Difference between revisions of "Part:BBa K218005"

Latest revision as of 20:48, 21 October 2009

LuxOU generator with terminator upstream

Vibrio harveyi luminescence regulatory protein LuxO (GenBank accession# P0C5S5) and phosphorelay protein LuxU (GenBank accession# AAD12737) under control of TetR repressible promoter with upstream terminator. This LuxOU-B0015 construct is an intermediate in the construction of the AI-2 signalling circuit (LuxPQ-B0015-R0040-LuxOU-B0015; Part:BBa_K218006). This circuit was constructed with Part:BBa_K218003 and Part:BBa_K218004. For usage and biology of this intermediate, refer to Part:BBa_K218001. This construct allows for constitutive expression of LuxOU while insuring that the cloning of any upstream gene is sufficiently insulated.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1053
Illegal SapI.rc site found at 1015

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 <partinfo>BBa_K218005 short</partinfo>
-Vibrio harveyi luminescence regulatory protein LuxO (GenBank accession# P0C5S5) and phosphorelay protein LuxU (GenBank accession# AAD12737) under control of TetR repressible promoter with upstream terminator. This LuxOU-B0015 construct is an intermediate in the construction of the AI-2 signalling circuit (LuxPQ-B0015-R004-LuxOU-B0015; [[Part:BBa_K218006]]). This circuit was constructed with [[Part:BBa_K218003]] and [[Part:BBa_K218004]]. For usage and biology of this intermediate, refer to [[Part:BBa_K218001]]. This construct allows for constitutive expression of LuxOU while insuring that the cloning of any upstream gene is sufficiently insulated.
+Vibrio harveyi luminescence regulatory protein LuxO (GenBank accession# P0C5S5) and phosphorelay protein LuxU (GenBank accession# AAD12737) under control of TetR repressible promoter with upstream terminator.
+This LuxOU-B0015 construct is an intermediate in the construction of the AI-2 signalling circuit (LuxPQ-B0015-R0040-LuxOU-B0015; [[Part:BBa_K218006]]). This circuit was constructed with [[Part:BBa_K218003]] and [[Part:BBa_K218004]]. For usage and biology of this intermediate, refer to [[Part:BBa_K218001]]. This construct allows for constitutive expression of LuxOU while insuring that the cloning of any upstream gene is sufficiently insulated.
 <!-- Add more about the biology of this part here