Difference between revisions of "Part:BBa K209403"

 
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<partinfo>BBa_K209403 short</partinfo>
 
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GPCR N terminal tags flanked by AarI AB sites.
  
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===Usage and Biology===
 
===Usage and Biology===
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Intended to be used as an N-terminal addition to G protein-coupled receptors.  The signal sequence from prolactin is reported to increase surface expression of GPCRs and is cleaved off leaving the free FLAG epitope, which is recognized by the M1 antibody. 
  
 
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Revision as of 20:45, 21 October 2009

AarI A-B part, signal sequence-FLAG

GPCR N terminal tags flanked by AarI AB sites.

Usage and Biology

Intended to be used as an N-terminal addition to G protein-coupled receptors. The signal sequence from prolactin is reported to increase surface expression of GPCRs and is cleaved off leaving the free FLAG epitope, which is recognized by the M1 antibody.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 128
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 84