Difference between revisions of "Part:BBa K5109060:Design"
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| + | We designed primers forward and reverse overlapping the restriction site, both of them with a single mismatch on the second T of the site "CATATG". | ||
| + | Primer forward: 5’ – CCGCATAAGGTGCACTCTCAGTACAAT – 3’ | ||
| + | Primer reverse: 5’ – GTGCACCTTATGCGGTGTGAAATACCG – 3’ | ||
| + | |||
| + | After the mutagenesys, the restriction site was changed from CATATG to CATAAG. | ||
Revision as of 20:19, 28 September 2024
We designed primers forward and reverse overlapping the restriction site, both of them with a single mismatch on the second T of the site "CATATG".
Primer forward: 5’ – CCGCATAAGGTGCACTCTCAGTACAAT – 3’ Primer reverse: 5’ – GTGCACCTTATGCGGTGTGAAATACCG – 3’
After the mutagenesys, the restriction site was changed from CATATG to CATAAG.