Difference between revisions of "Part:BBa K180003"

 
 
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__NOTOC__
 
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<partinfo>BBa_K180003 short</partinfo>
 
<partinfo>BBa_K180003 short</partinfo>
  
 
As per BBa_K180002, omitting the RBS and STOP biobrick parts (B0034 and B0015) for speed and ease of construction.
 
As per BBa_K180002, omitting the RBS and STOP biobrick parts (B0034 and B0015) for speed and ease of construction.
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The lacIq sequence preceding the tac promoter ensures that tac pR is in the 'OFF' state naturally. Green fluorescent protein is produced if IPTG successfully induces the promoter, demonstrating the regulatory effect of Lac upon the promoter.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 20:45, 21 October 2009

PTAC testing plasmid (GFP) - basic

As per BBa_K180002, omitting the RBS and STOP biobrick parts (B0034 and B0015) for speed and ease of construction.

The lacIq sequence preceding the tac promoter ensures that tac pR is in the 'OFF' state naturally. Green fluorescent protein is produced if IPTG successfully induces the promoter, demonstrating the regulatory effect of Lac upon the promoter.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2097