Difference between revisions of "Part:BBa K243003:Design"

(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
−
Designed with Biobrick pre-and suffix for fusion proteins according the [https://parts.igem.org/Assembly_standard_25 RFC 25].<br>
+
Designed with Biobrick pre-and suffix for fusion proteins according to the [https://parts.igem.org/Assembly_standard_25 RFC 25].<br>
 
[https://parts.igem.org/Image:DigA.txt  Commented GenBank file]
 
[https://parts.igem.org/Image:DigA.txt  Commented GenBank file]
  
 
===Source===
 
===Source===
  
−
Planed and designed by Team Freiburg Bioware09 andsynthesized by purimex.<br>
+
Planned and designed by Team Freiburg Bioware09 and synthesized by purimex.<br>
  
 
===References===
 
===References===

Latest revision as of 20:44, 21 October 2009

Digoxigenin binding protein (DigA)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 248
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Designed with Biobrick pre-and suffix for fusion proteins according to the RFC 25.
Commented GenBank file

Source

Planned and designed by Team Freiburg Bioware09 and synthesized by purimex.

References

Holtke HJ, Ankenbauer W, Muhlegger K, Rein R, Sagner G, Seibl R, Walter T. (1995)
The digoxigenin (DIG) system for non-radioactive labelling and detection of nucleic acids--an overview. Cell Mol Biol. 41(7):883-905.

Roche Applied Science (2002) DIG Application Manual for Nonradioactive In Situ Hybridization (3rdedition)

Moter A, Göbel UB (2000) Fluorescence in situ hybridisation (FISH) for direct visualization of microorganism, J Microbiol Meth. 41: 85-112

http://www.thermo.com/eThermo/CMA/PDFs/Various/File_29495.pdf (image)