Difference between revisions of "Part:BBa K177050:Design"
(→Source) |
|||
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K177050 short</partinfo> | <partinfo>BBa_K177050 short</partinfo> | ||
| Line 13: | Line 12: | ||
===Source=== | ===Source=== | ||
| − | + | <partinfo>BBa_C0051</partinfo> DNA was used as themplate to site directed mutagenesis. Our aim was changing T<sub>450</sub>->C to remove premature stop codon and introducion of cIts857 mutation (TTTTGCAAGCAA -> | |
| − | + | TTTTGtAAGCAA) to make this variant of cI thermosensitive. | |
| − | + | ||
===References=== | ===References=== | ||
Latest revision as of 20:43, 21 October 2009
cI ts
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Source
BBa_C0051 DNA was used as themplate to site directed mutagenesis. Our aim was changing T450->C to remove premature stop codon and introducion of cIts857 mutation (TTTTGCAAGCAA -> TTTTGtAAGCAA) to make this variant of cI thermosensitive.