Difference between revisions of "Part:BBa K218005"

Revision as of 20:41, 21 October 2009

LuxOU generator with terminator upstream

Vibrio harveyi luminescence regulatory protein LuxO (GenBank accession# P0C5S5) and phosphorelay protein LuxU (GenBank accession# AAD12737) under control of TetR repressible promoter with upstream terminator. This LuxOU-B0015 construct is an intermediate in the construction of the AI-2 signalling circuit (LuxPQ-B0015-R004-LuxOU-B0015; Part:BBa_K218006). This circuit was constructed with Part:BBa_K218003 and Part:BBa_K218004. For usage and biology of this intermediate, refer to Part:BBa_K218001. This construct allows for constitutive expression of LuxOU while insuring that the cloning of any upstream gene is sufficiently insulated.

being able to clone any gene upstream of the B0015 terminator.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1053
Illegal SapI.rc site found at 1015

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 <partinfo>BBa_K218005 short</partinfo>
-Vibrio harveyi luminescence regulatory protein LuxO (GenBank accession# P0C5S5) and phosphorelay protein LuxU (GenBank accession# AAD12737) under control of TetR repressible promoter with upstream terminator. This LuxOU-B0015 construct is an intermediate in the construction of the AI-2 signalling circuit (LuxPQ-B0015-R004-LuxOU-B0015; [[Part:BBa_K218006]]). This circuit was constructed with [[Part:BBa_K218003]] and [[Part:BBa_K218004]]. For usage and biology of this intermediate, refer to [[Part:BBa_K218001]]. This construct allows for constitutive expression of LuxOU while being able to clone any gene upstream of the B0015 terminator.
+Vibrio harveyi luminescence regulatory protein LuxO (GenBank accession# P0C5S5) and phosphorelay protein LuxU (GenBank accession# AAD12737) under control of TetR repressible promoter with upstream terminator. This LuxOU-B0015 construct is an intermediate in the construction of the AI-2 signalling circuit (LuxPQ-B0015-R004-LuxOU-B0015; [[Part:BBa_K218006]]). This circuit was constructed with [[Part:BBa_K218003]] and [[Part:BBa_K218004]]. For usage and biology of this intermediate, refer to [[Part:BBa_K218001]]. This construct allows for constitutive expression of LuxOU while insuring that the cloning of any upstream gene is sufficiently insulated.
+being able to clone any gene upstream of the B0015 terminator.
 <!-- Add more about the biology of this part here