Difference between revisions of "Part:BBa C0051:Experience"
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Our miniprep, double digest and single digest worked as expected. We used this part for further cloning which was successful. The sequencing was correct. However we can not confirm that this part was working as we did not test it's functionality.
 
Our miniprep, double digest and single digest worked as expected. We used this part for further cloning which was successful. The sequencing was correct. However we can not confirm that this part was working as we did not test it's functionality.
 
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<partinfo>BBa_C0051 AddReview 1</partinfo>
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<I>Michael Lower Warsaw 2009 Team</I>
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Our copy of <partinfo>BBa_C0051</partinfo> was somewhat defective and wasn't able to repress 'cI lam' promoter efficiently enough.
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We have sequenced this brick (results are in 'sequence analysis' tab) and it turned out that it has point mutation C->T at position 450 of cI CDS which leads to formation of premature stop codon at amino acid position 360. Additionally it has two other point mutations S<sub>504</sub>->N and S<sub>786</sub>->C. It leads to truncated nonfunctional protein. We have constructed working version of this brick <partinfo>BBa_K177050</partinfo>.
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Revision as of 20:39, 21 October 2009

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_C0051

This part should have a history. Please add content if you have used it.

User Reviews

UNIQa49d51147f00115b-partinfo-00000000-QINU

••••

Aberdeen_Scotland 2009

Our miniprep, double digest and single digest worked as expected. We used this part for further cloning which was successful. The sequencing was correct. However we can not confirm that this part was working as we did not test it's functionality.

•

Michael Lower Warsaw 2009 Team

Our copy of BBa_C0051 was somewhat defective and wasn't able to repress 'cI lam' promoter efficiently enough.

We have sequenced this brick (results are in 'sequence analysis' tab) and it turned out that it has point mutation C->T at position 450 of cI CDS which leads to formation of premature stop codon at amino acid position 360. Additionally it has two other point mutations S504->N and S786->C. It leads to truncated nonfunctional protein. We have constructed working version of this brick BBa_K177050.

UNIQa49d51147f00115b-partinfo-00000005-QINU