Difference between revisions of "Part:BBa K5117042"

 
Line 11: Line 11:
 
- <i>bglB</i> gene of <i>Paenibacillus polymyxa</i> encoding a beta-glucosidase (EC 3.2.1.21),
 
- <i>bglB</i> gene of <i>Paenibacillus polymyxa</i> encoding a beta-glucosidase (EC 3.2.1.21),
  
−
addition of a long flexible linker (L1) downstream of the coding sequence encoding the amino acids (GGGGS)4 (BBa_K5117030),
+
addition of a long flexible linker (L2) downstream of the coding sequence encoding the amino acids (GGGGS)4 (BBa_K5117030),
  
 
- <i>cotY</i> gene of <i>Bacillus subtilis</i> (BBa_K5117022),
 
- <i>cotY</i> gene of <i>Bacillus subtilis</i> (BBa_K5117022),

Revision as of 19:02, 28 September 2024


PcotYZ-BsRBS-PpBglB-L2-CotY-B0014

This part serves as transcriptional unit composed of:

- promoter PcotYZ of Bacillus subtilis (BBa_K5117021),

- ribosome binding site of Bacillus subtilis (BBa_K5117000),

- bglB gene of Paenibacillus polymyxa encoding a beta-glucosidase (EC 3.2.1.21),

addition of a long flexible linker (L2) downstream of the coding sequence encoding the amino acids (GGGGS)4 (BBa_K5117030),

- cotY gene of Bacillus subtilis (BBa_K5117022),

- bidirectional terminator B0014 (BBa_B0014).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1627
  • 1000
    COMPATIBLE WITH RFC[1000]