Difference between revisions of "Part:BBa K5097013"

Revision as of 18:41, 28 September 2024

BBa_K5097013 6X His tag with AGVG linker

Sequence and Features

Assembly Compatibility:

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 __NOTOC__
 <partinfo>BBa_K5097013 short</partinfo>
-This basic part codes for an 6X Histidine tag with a N-terminal AGVG linker and C terminal double stop codon.  The part is intended for use in protein purification using IMAC (Immobilized Metal ion Affinity Chromatography) purification.  This part can be added to the C-terminal of a coding sequence (with the native stop codon removed), which will add the flexible AGVG linker and then a 6X Histidine tag.
 <!-- Add more about the biology of this part here
 ===Usage and Biology===
+This basic part codes for an 6X Histidine tag with a N-terminal AGVG linker and C terminal double stop codon.  The part is intended for use in protein purification using IMAC (Immobilized Metal ion Affinity Chromatography) purification.  This part can be added to the C-terminal of a coding sequence (with the native stop codon removed), which will add the flexible AGVG linker and then a 6X Histidine tag.
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