Difference between revisions of "Part:BBa K245068:Design"

(→‎Source)
(→‎Design Notes)
 
Line 6: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
The part is inserted in BB-NIC-II-HisN vector (BBa_K245005) and can be assembled with other parts according to assembly standard 37.  Composite parts were assembled (iGEM 2009, team Slovenia) by cutting front inserts with EcoRI and BspEI and front vectors with EcoRI and NgoMIV,  thus forming RFC 37 Scar Sequence (BBa_K245009) beween separate parts.
+
The part is inserted in BB-NIC-II-HisN vector (BBa_K245005) and can be assembled with other parts according to assembly standard 37.  Composite parts were assembled (iGEM 2009, team Slovenia) by cutting front inserts with EcoRI and BspEI and front vectors with EcoRI and NgoMIV,  thus forming Scar Sequence (BBa_K245009) beween separate parts according to assembly standard 37.
  
 
===Source===
 
===Source===

Latest revision as of 20:34, 21 October 2009

P1-P3-P5


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part is inserted in BB-NIC-II-HisN vector (BBa_K245005) and can be assembled with other parts according to assembly standard 37. Composite parts were assembled (iGEM 2009, team Slovenia) by cutting front inserts with EcoRI and BspEI and front vectors with EcoRI and NgoMIV, thus forming Scar Sequence (BBa_K245009) beween separate parts according to assembly standard 37.

Source

Composed from P1 basic part and P3-P5 intermediate part according to assembly standard 37.

References