Difference between revisions of "Part:BBa K5107001:Design"

 
m
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
Chnage the nt that define the SalI restriction site
+
As the system should be transcriptionally functional when the hormone is not present in the solution, we add the di-nucleotides GA between the T7 promoter and the HRE in order to keep high transcription level(extra Gs in the 3' end of the promoter)[1].
  
  
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===Source===
 
===Source===
  
−
Extract by PCR amplification from the plasmid pRR-MR-5Z
+
The part is synthesised as member of the whole device we used in our cell free system obtaining as g-block from IDT.
  
 
===References===
 
===References===
 +
1.Conrad, T., Plumbom, I., Alcobendas, M., Vidal, R., & Sauer, S. (2020). Maximizing transcription of nucleic acids with efficient T7 promoters. Communications Biology, 3(1). https://doi.org/10.1038/s42003-020-01167-x

Revision as of 18:26, 28 September 2024


ERE minimal


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

As the system should be transcriptionally functional when the hormone is not present in the solution, we add the di-nucleotides GA between the T7 promoter and the HRE in order to keep high transcription level(extra Gs in the 3' end of the promoter)[1].


Source

The part is synthesised as member of the whole device we used in our cell free system obtaining as g-block from IDT.

References

1.Conrad, T., Plumbom, I., Alcobendas, M., Vidal, R., & Sauer, S. (2020). Maximizing transcription of nucleic acids with efficient T7 promoters. Communications Biology, 3(1). https://doi.org/10.1038/s42003-020-01167-x