Difference between revisions of "Part:BBa K5107000:Design"

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===Design Notes===
 
===Design Notes===
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As the system should be transcriptionally functional when the hormone is not present in the solution, we add the di-nucleotides GA between the T7 promoter and the HRE in order to keep high transcription level(extra Gs in the 3' end of the promoter).
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As the system should be transcriptionally functional when the hormone is not present in the solution, we add the di-nucleotides GA between the T7 promoter and the HRE in order to keep high transcription level(extra Gs in the 3' end of the promoter)[1].
  
  
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===Source===
 
===Source===
  
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The part is synthesised as g-block part of the the whole device we used in our cell free system by IDT.
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The part is synthesised as member of the whole device we used in our cell free system obtaining as g-block from IDT.
  
 
===References===
 
===References===
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1.Miller, C. A., Tan, X., Wilson, M., Bhattacharyya, S., & Ludwig, S. (2010). Single plasmids expressing human steroid hormone receptors and a reporter gene for use in yeast signaling assays. Plasmid, 63(2), 73–78. https://doi.org/10.1016/j.plasmid.2009.11.003
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1.Conrad, T., Plumbom, I., Alcobendas, M., Vidal, R., & Sauer, S. (2020). Maximizing transcription of nucleic acids with efficient T7 promoters. Communications Biology, 3(1). https://doi.org/10.1038/s42003-020-01167-x
  
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2.Tan, M. E., Li, J., Xu, H. E., Melcher, K., & Yong, E. (2014). Androgen receptor: structure, role in prostate cancer and drug discovery. Acta Pharmacologica Sinica, 36(1), 3–23. https://doi.org/10.1038/aps.2014.18
 
  
 
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Revision as of 18:24, 28 September 2024


HRE_minimal


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

As the system should be transcriptionally functional when the hormone is not present in the solution, we add the di-nucleotides GA between the T7 promoter and the HRE in order to keep high transcription level(extra Gs in the 3' end of the promoter)[1].


Source

The part is synthesised as member of the whole device we used in our cell free system obtaining as g-block from IDT.

References

1.Conrad, T., Plumbom, I., Alcobendas, M., Vidal, R., & Sauer, S. (2020). Maximizing transcription of nucleic acids with efficient T7 promoters. Communications Biology, 3(1). https://doi.org/10.1038/s42003-020-01167-x


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