Difference between revisions of "Part:BBa K218005:Design"

 
(→‎Design Notes)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K218005 short</partinfo>
 
<partinfo>BBa_K218005 short</partinfo>
Line 7: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
design
+
No RBS was cloned into this intermediate because the LuxOU operons already has an internal RBS. Although there is an internal terminator as well, we have constructed the strong terminator B0015 after this coding sequence in order to provide further insulation of the circuit. The B0015 terminator was cloned upstream of the LuxOU generator because this intermediate will allow for subsequent cloning and insulation of LuxPQ upstream of this terminator.
−
 
+
−
 
+
  
 
===Source===
 
===Source===

Revision as of 20:32, 21 October 2009

LuxOU generator with terminator upstream


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1053
    Illegal SapI.rc site found at 1015


Design Notes

No RBS was cloned into this intermediate because the LuxOU operons already has an internal RBS. Although there is an internal terminator as well, we have constructed the strong terminator B0015 after this coding sequence in order to provide further insulation of the circuit. The B0015 terminator was cloned upstream of the LuxOU generator because this intermediate will allow for subsequent cloning and insulation of LuxPQ upstream of this terminator.

Source

source

References