Difference between revisions of "Part:BBa K5246029:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | <p>Plasmids were cloned using Golden Gate assembly, inserting the <i>HfsL</i> gene into the pRSFDuet backbone.</p> | |
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===Source=== | ===Source=== | ||
Latest revision as of 18:08, 28 September 2024
CB2/CB2A HfsL Glycosyltransferase, 6xHis tag for purification
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 265
Illegal NgoMIV site found at 408
Illegal NgoMIV site found at 639 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Plasmids were cloned using Golden Gate assembly, inserting the HfsL gene into the pRSFDuet backbone.
Source
Caulobacter crescentus (Caulobacter vibroides) CB2 (DSM 4727, ATCC 15252) Caulobacter crescentus (Caulobacter vibroides) CB2A (DSM 25117)