Difference between revisions of "Part:BBa K5117035"
Line 9: Line 9:
 
- ribosome binding site of <i>Bacillus subtilis</i> (BBa_K5117000),  
 
- ribosome binding site of <i>Bacillus subtilis</i> (BBa_K5117000),  
  
−
- <i>eglS</i> gene of <i>Bacillus subtilis</i> without signal peptide, encoding an endoglucanase (EC 3.2.1.4),
+
- <i>eglS</i> gene of <i>Bacillus subtilis</i> without signal peptide (BBa_K5117023), encoding an endoglucanase (EC 3.2.1.4),
  
−
downstream of the coding sequence, a short flexible linker (L1) has been added encoding the amino acids GA (BBa_K5117023)
+
addition of a short flexible linker (L1) downstream of the coding sequence encoding the amino acids GA
  
 
- <i>cotY</i> gene of <i>Bacillus subtilis</i> (BBa_K5117022),
 
- <i>cotY</i> gene of <i>Bacillus subtilis</i> (BBa_K5117022),

Revision as of 18:07, 28 September 2024


PcotYZ-BsRBS-BsEglS-L1-CotY-B0014

This part serves as transcriptional unit composed of:

- promoter PcotYZ of Bacillus subtilis (BBa_K5117021),

- ribosome binding site of Bacillus subtilis (BBa_K5117000),

- eglS gene of Bacillus subtilis without signal peptide (BBa_K5117023), encoding an endoglucanase (EC 3.2.1.4),

addition of a short flexible linker (L1) downstream of the coding sequence encoding the amino acids GA

- cotY gene of Bacillus subtilis (BBa_K5117022),

- bidirectional terminator B0014 (BBa_B0014)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1642
    Illegal AgeI site found at 726
  • 1000
    COMPATIBLE WITH RFC[1000]