Difference between revisions of "Part:BBa K5117035"

Revision as of 18:05, 28 September 2024

PcotYZ-BsRBS-BsEglS-L1-CotY-B0014

This part serves as transcriptional unit composed of:

- promoter PcotYZ of Bacillus subtilis (BBa_K5117021),

- ribosome binding site of Bacillus subtilis (BBa_K5117000),

- eglS gene of Bacillus subtilis without signal peptide, encoding an endoglucanase (EC 3.2.1.4),

downstream of the coding sequence, a short flexible linker (L1) has been added encoding the amino acids GA (BBa_K5117023)

- cotY gene of Bacillus subtilis (BBa_K5117022),

- bidirectional terminator B0014 (BBa_B0014)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1642
Illegal AgeI site found at 726
1000
COMPATIBLE WITH RFC[1000]

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 This part serves as transcriptional unit composed of:
 - promoter P<i>cotYZ</i> of <i>Bacillus subtilis</i> (BBa_K5117021),
 - ribosome binding site of <i>Bacillus subtilis</i> (BBa_K5117000),
 - <i>eglS</i> gene of <i>Bacillus subtilis</i> without signal peptide, encoding an endoglucanase (EC 3.2.1.4),
 downstream of the coding sequence, a short flexible linker (L1) has been added encoding the amino acids GA (BBa_K5117023)
 - <i>cotY</i> gene of <i>Bacillus subtilis</i> (BBa_K5117022),
 - bidirectional terminator B0014 (BBa_B0014)
 <!-- Add more about the biology of this part here