Difference between revisions of "Part:BBa K5097009"
Line 8: Line 8:
 
[[File:T--Suny Oneonta-t7-virus-structure.jpg|200px|frame|center|Figure 1: A labeled visual detailing the various structures of a T7-like phage (Kemp et al., 2005)]]
 
[[File:T--Suny Oneonta-t7-virus-structure.jpg|200px|frame|center|Figure 1: A labeled visual detailing the various structures of a T7-like phage (Kemp et al., 2005)]]
  
−
[[File: putrescine-pathway.jpg|200px|frame|center|Figure 1: Conversion of Arginine to Putrescine. Figure adapted from reference 3 (Charlier et al., 2019)]]
+
[[File:T-oneonta-putrescine-pathway.jpg|200px|frame|center|Figure 1: Conversion of Arginine to Putrescine. Figure adapted from reference 3 (Charlier et al., 2019)]]
  
  

Revision as of 18:04, 28 September 2024


SpeA (arginine decarboxylase)

Usage and Biology

This specific part codes for SpeA gene from E.coli. It expresses the enzyme arginine decarboxylase (Shaibe et al., 1985). This enzyme catalyzes the conversion of L-arginine into agmatine and carbon dioxide, the first step in the metabolic pathway that converts arginine into putrescine (Morris et al., 1969). The 2024 Oneonta iGEM designed this sequence for use in a base producing circuit. This part when co-expressed with SpeB (BBa_K0597010) will construct this pathway and produce putrescine from arginine.

Figure 1: A labeled visual detailing the various structures of a T7-like phage (Kemp et al., 2005)
File:T-oneonta-putrescine-pathway.jpg
Figure 1: Conversion of Arginine to Putrescine. Figure adapted from reference 3 (Charlier et al., 2019)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1389
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1167
  • 1000
    COMPATIBLE WITH RFC[1000]