Difference between revisions of "Part:BBa K209400"

Revision as of 20:25, 21 October 2009

AarI B-C part, hM4D

hM4 DREADD flanked by AarI BC sites.

Usage and Biology

This is an engineered version of the M4 muscarinic receptor that can be activated by the otherwise inert small molecule, clozapine-N-oxide.

Reference: Evolving the lock to fit the key to create a family of G protein-coupled receptors potently activated by an inert ligand. Armbruster BN, Li X, Pausch MH, Herlitze S, Roth BL. Proc Natl Acad Sci U S A. 2007 Mar 20;104(12):5163-8. Epub 2007 Mar 2.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 184
Illegal PstI site found at 979
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1215
Illegal PstI site found at 184
Illegal PstI site found at 979
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 184
Illegal PstI site found at 979
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 184
Illegal PstI site found at 979
Illegal NgoMIV site found at 1086
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K209400 short</partinfo>
@@ Line 5: / Line 4: @@
 hM4 DREADD flanked by AarI BC sites.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+ This is an engineered version of the M4 muscarinic receptor that can be activated by the otherwise inert small molecule, clozapine-N-oxide.
+Reference:
+Evolving the lock to fit the key to create a family of G protein-coupled receptors potently activated by an inert ligand. Armbruster BN, Li X, Pausch MH, Herlitze S, Roth BL. Proc Natl Acad Sci U S A. 2007 Mar 20;104(12):5163-8. Epub 2007 Mar 2.
 <!-- -->