Difference between revisions of "Part:BBa K218005"
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This LuxOU-B0015 construct is an intermediate in the construction of the AI-2 signalling circuit (LuxPQ-B0015-R004-LuxOU-B0015; [[Part:BBa_K218006]]). This circuit was constructed with [[Part:BBa_K218003]] and [[Part:BBa_K218004]].  
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This LuxOU-B0015 construct is an intermediate in the construction of the AI-2 signalling circuit (LuxPQ-B0015-R004-LuxOU-B0015; [[Part:BBa_K218006]]). This circuit was constructed with [[Part:BBa_K218003]] and [[Part:BBa_K218004]]. For usage and biology of this intermediate, refer to [[Part:BBa_K218001]]. This construct allows for constitutive expression of LuxOU while being able to clone any gene upstream of the B0015 terminator.
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For usage and biology of this intermediate, refer to [[Part:BBa_K218001]].
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Revision as of 20:25, 21 October 2009

LuxOU generator with terminator upstream


This LuxOU-B0015 construct is an intermediate in the construction of the AI-2 signalling circuit (LuxPQ-B0015-R004-LuxOU-B0015; Part:BBa_K218006). This circuit was constructed with Part:BBa_K218003 and Part:BBa_K218004. For usage and biology of this intermediate, refer to Part:BBa_K218001. This construct allows for constitutive expression of LuxOU while being able to clone any gene upstream of the B0015 terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1053
    Illegal SapI.rc site found at 1015