Difference between revisions of "Part:BBa K218003"
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<partinfo>BBa_K218003 short</partinfo>
 
<partinfo>BBa_K218003 short</partinfo>
  
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Vibrio harveyi luminescence regulatory protein LuxO (GenBank accession# P0C5S5) and phosphorelay protein LuxU (GenBank accession# AAD12737) under control of TetR repressible promoter with upstream terminator. This LuxOU-B0015 construct is an intermediate in the construction of the AI-2 signalling circuit (LuxPQ-B0015-R004-LuxOU-B0015; [[Part:BBa_K218006]]). For usage and biology of this intermediate, refer to [[Part:BBa_K218001]]. This construct allows for constitutive expression of LuxOU while being able to clone any gene upstream.
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Vibrio harveyi luminescence regulatory protein LuxO (GenBank accession# P0C5S5) and phosphorelay protein LuxU (GenBank accession# AAD12737) under control of TetR repressible promoter with upstream terminator. This LuxOU-B0015 construct is an intermediate in the construction of the AI-2 signalling circuit (LuxPQ-B0015-R004-LuxOU-B0015; [[Part:BBa_K218006]]). For usage and biology of this intermediate, refer to [[Part:BBa_K218001]].  
  
  

Revision as of 20:24, 21 October 2009

LuxOU from Vibrio harveyi followed by terminator

Vibrio harveyi luminescence regulatory protein LuxO (GenBank accession# P0C5S5) and phosphorelay protein LuxU (GenBank accession# AAD12737) under control of TetR repressible promoter with upstream terminator. This LuxOU-B0015 construct is an intermediate in the construction of the AI-2 signalling circuit (LuxPQ-B0015-R004-LuxOU-B0015; Part:BBa_K218006). For usage and biology of this intermediate, refer to Part:BBa_K218001.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 854
    Illegal SapI.rc site found at 816